Table 2.

Quantitative PCR specifics

Putative Gene Annotation	Microarray identifier	EST Accession No.	Sequence (5′-3′)#	Amplicon length (bp)	Amplification Efficiency (%)
ZPC1	TTC00305	EC091965	F – CATACCACCCTTCACCCATC	212	102
			R – GCTCCACACTAGCCCATGAT
Vitelline egg envelope gamma	TTC00056	EC091692	F – GCTTGCATGTGTCAGGCTTA	198	94
			R – GGAGAATGGCTTGACTGCTC
Aveolin	TTC00935	EC092671	F – GCTTCTGCTGCTCTTCGTCT	198	90
			R – AACAACACCTGAGGCAGGAC
Choriogenin L	TTC04136	EG630294	F – GAGCAGTCAAGCCATTCTCC	230	94
			R – CGTCAATCTCAGTGGCTGAA
Cathepsin S	TTC00230	EC091881	F – GGATCGACACTGGGAACTGT	297	92
			R – CCCTCCAGTCCAGTGATTGT
Aquaporin 1	TTC00180	EC091829	F – CCTGTTTCGCAGTCTTGGAT	191	96
			R – GGTCGGGGTAGGAATCATTT
Tmc6-related protein 1	TTC04625	EG630885	F – CCGGTTTCTCCTCACCAATA	135	91
			R – TTGTGCGTGACATTCCTGAT
Fatty acid-binding protein, adipocyte	TTC00964	EC092703	F – ACTGCAATGACCGAAAGACC	175	97
			R – CCTCCTTTCCGTAGGTCCTC
Epididymal secretory protein E1 precursor	TTC00209	EC091860	F – GCTTGATGGGATTCACCTGT	215	94
			R – CGATTATTCCCATGGACCAC
Synaptonemal complex protein 3	TTC02745	EC918114	F – AAGAGCTGAGCGGTTCAGAG	264	91
			R – TGACCGTGGTAGTTGTTCCA
T-complex-associated testis-expressed protein 1	TTC02749	EC918118	F – TGCTGGTGAAACACCTCTTG	208	92
			R – AGGGACAAAAGGGTGGAGTT
Brain-type fatty acid binding protein	TTC05153	EG999669	F – CCTACACCTGATGACCGACA	212	98
			R – GCTGGGATGATTTGCTCATT
Intestinal fatty acid-binding protein	TTC05128	EG999641	F – CGCAGCGAGAATTATGACAA	244	95
			R – AGCATGTCACCCTCCATCTC

^#F refers to the forward primer while R indicates the reverse primer.