Table 1.
Phenotypic analysis of tumor infiltrating iNOS+P1a and negative P4 subpopulations
| P4 | P1a | |
|---|---|---|
| Monocyte/PMN subset marker/Migration |
|
|
| Ly6C |
+ |
-/+ |
| Ly6G |
++ |
- |
| CXCR2 |
++ |
- |
| CCR3 |
+ |
- |
| Siglec-F |
- |
-/+ |
| F4/80 |
- |
++ |
| CXCR4 |
- |
- |
| CD62L |
- |
- |
| CD49d |
++ |
++ |
| Adhesion/Activation Molecules |
|
++ |
| CD44 |
++ |
++ |
| CD43 |
++ |
- |
| CD103 |
- |
- |
| CD138 |
++ |
++ |
| CD54 (ICAM-1) |
++ |
++ |
| Antigen presentation |
|
- |
| MHC I |
++ |
- |
| MHC II |
- |
- |
| CD86 (B7-2) |
+ |
+ |
| CD11c |
- |
- |
| Differentiation |
|
- |
| CD119 (IFNGR1) |
++ |
- |
| CD124 (IL-4Ra) |
++ |
+ |
| CD115 (CSFR1) |
- |
- |
| CD309 (VEGFR2) |
+ |
+ |
| Potential T-cell suppressive marker |
|
|
| B7H1 (PD-L1) |
+ |
+ |
| B7DC (PD-L2) |
- |
- |
| FASL |
+ |
- |
| Pattern recognition receptor |
|
- |
| CD14 |
- |
- |
| TLR2 | + | + + |
Expression of the indicated cell surface markers was evaluated on gated SSChi CD11bhi Gr-1dim F4/80+ P1a and Gr-1hi CD11bhi F4/80- (P4) cells. “-” indicates no expression and “+”, “++”, increasing amount of expression to isotype-matched controls.