Figure 4.

Diurnal and circadian expression of flowering genes in the spring cultivar Scarlett (ppd-H1 ) and the introgression line S42 IL-107 (Ppd-H1 ) under long day and free running conditions. Scarlett (solid line) and S42-IL107 (dashed line) were grown under long day conditions (16 hours light - left panel) for two weeks and then released in continuous light (right panel). Transcript accumulation was measured at two hour intervals (one-hour interval at the end of the day and beginning of the night/subjective nights) by qRT-PCR analysis of specific genes and normalized to HvActin. Values represent the average of two technical replicates ± standard error. Black and grey bars indicate objective and subjective nights.