Table 2.
Expression in subcutaneous adipose tissue of ceramide-metabolising enzymes in relation to adipose tissue inflammation
|
Gene |
ABI assay ID |
Ct value |
Less inflamed adipose tissue, normal liver fat |
More inflamed adipose tissue, high liver fat |
P |
|---|---|---|---|---|---|
| n = 10 | n = 10 | ||||
| SPTLC1 |
Hs00272311_m1 |
27.2 |
0.99 ± 0.04 |
1.05 ± 0.06 |
0.20 |
| SPTLC2 |
Hs00191585_m1 |
26.8 |
0.97 ± 0.07 |
1.00 ± 0.05 |
0.39 |
| DEGS1 |
Hs00186447_m1 |
25.9 |
0.99 ± 0.05 |
1.07 ± 0.05 |
0.11 |
| LASS1 |
Hs00242151_m1 |
33.2 |
9.07 ± 3.12 |
9.22 ± 1.63 |
0.48 |
| LASS4 |
Hs00226114_m1 |
27.1 |
1.08 ± 0.10 |
1.07 ± 0.16 |
0.49 |
| LASS6 |
Hs00826756_m1 |
28.8 |
0.87 ± 0.07 |
0.89 ± 0.07 |
0.42 |
| ASAH1 |
Hs00602774_m1 |
24.3 |
1.16 ± 0.10 |
1.47 ± 0.13 |
0.03 |
| UGCG |
Hs00234293_m1 |
28.2 |
0.91 ± 0.08 |
0.96 ± 0.06 |
0.34 |
| SGMS1 |
Hs00380453_m1 |
28.0 |
1.01 ± 0.05 |
1.10 ± 0.06 |
0.12 |
| SGMS2 |
Hs00398067_m1 |
30.6 |
2.09 ± 0.27 |
2.57 ± 0.32 |
0.13 |
| SMPD1 |
Hs00609415_m1 |
26.6 |
1.51 ± 0.10 |
1.85 ± 0.10 |
0.01a |
| SMPD2 |
Hs00162006_m1 |
29.8 |
1.13 ± 0.06 |
1.26 ± 0.06 |
0.08 a |
| SMPD3 |
Hs00218713_m1 |
33.8 |
1.33 ± 0.13 |
1.76 ± 0.18 |
0.05a |
| SMPD4 |
Hs00215775_m1 |
29.1 |
1.57 ± 0.09 |
1.52 ± 0.09 |
0.35 |
| CERK |
Hs00368483_m1 |
29.3 |
1.57 ± 0.15 |
1.87 ± 0.10 |
0.06 |
| SPHK1 |
Hs00184211_m1 |
28.9 |
12.18 ± 1.49 |
17.64 ± 1.65 |
0.01 |
| CGT |
Hs00409961_m1 |
35.5 |
4.45 ± 1.29 |
3.45 ± 0.52 |
0.24 |
| CDH5 |
Hs00174344_m1 |
28.3 |
1.16 ± 0.14 |
1.43 ± 0.13 |
0.09 |
| SELE |
Hs00950401_m1 |
33.2 |
0.72 ± 0.15 |
2.45 ± 0.94 |
0.04 |
| VEGF |
Hs0090054_m1 |
26.4 |
4.13 ± 0.37 |
3.71 ± 0.52 |
0.25 |
| HIF-1α | Hs00153153_m1 | 27.6 | 1.39 ± 0.12 | 1.65 ± 0.08 | 0.02 |
Relative gene expression levels of genes involved in ceramide and sphingomyelin metabolism in subcutaneous adipose tissue of obese women with different degrees of adipose tissue inflammation are given. SPTLC: serine palmitoyl transferase long-chain; DEGS: dihydroceramide desaturase; LASS: LAG1 homolog (ceramide synthase); ASAH: N-acylsphingosine amidohydrolase (ceramidase); UGCG: UDP-glucose ceramide glucosyltransferase; SGMS: sphingomyelin synthase; SMPD: sphingomyelin phosphodiesterase (sphingomyelinase); CERK: ceramide kinase; SPHK: sphingosine kinase; CGT: ceramide glycosyl transferase; CDH5: cadherin 5 (vascular endothelium); SELE: selectin E; VEGF: vascular endothelial growth factor; HIF-1α: hypoxia-inducible factor 1α. Gene expression was normalized to housekeeping genes RPLP0 and TBP, which did not differ between the groups (for RPLP0: 1.94 ± 0.26 vs 2.11 ± 0.17, NS; for TBP: 3.96 ± 0.48 vs 3.97 ± 0.44, NS). Values are expressed as mean ± SEM. a Reported previously [3].