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. 2012 Aug 9;12:118. doi: 10.1186/1472-6882-12-118

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Copyright ©2012 Ogawa et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Purification of a biomarker candidate. Purification of the biomarker candidate peak at m/z 4223.1 involved using a combination of spin column and SDS-PAGE. A) Spectra of peak fractions eluted from the cation exchange spin column; before fractionation plasma, Fr. 2–2, Fr. 2–3, Fr. 2–4 and Fr. 2–5. The peaks were detected on a CM10 chip. The candidate peak is marked by an arrow. B) Each fraction was separated by SDS-PAGE and protein bands were visualized by silver staining. C) The band marked by an arrow in the Fr.2-3 lane was extracted from the gel and then analyzed by SELDI TOF-MS.