Figure 1. Host cell attachment and lysis by bacterial isolates. Attachment (top) and cell lysis (LDH release), (middle) caused by five different isolates each of ABC (A and E), P. aeruginosa (B and F), K. pneumoniae (C and G) and E. coli (D and H) tested on HeLa (blue) and 3T3 (red) cells. Values given are means ± standard error (n = 3) from a representative experiment performed in triplicate. Correlation between attachment and cytotoxicity on HeLa (●) and 3T3 (○) cells across different isolates (I–L). Pearson correlation coefficients (r values) and significance levels for correlation coefficients (p values) were used to analyze the correlation between strain attachment and cytotoxicity.