Figure 4. Visualization of anti-adhesion treatment of HeLa cells for representative examples from each group of bacterial isolate. HeLa cells were either left untreated (top row), pre-incubated with BL21-MAM7 (middle row) or pre-treated with bead-immobilized MAM7 (bottom row). Cells were then left uninfected (controls) (A, G and M) or infected with ABC isolate #1 (B, H and N), P. aeruginosa isolate #1 (C, I and O), P. aeruginosa isolate #3 (D, J and P), K. pneumoniae isolate #1 (E, K and Q) or E. coli isolate #5 (F, L and R) for 4 h. Cells were stained for actin (phalloidin-Alexa488, green) and DNA (Hoechst stain). Fluorescent latex beads are shown in yellow. Scalebar, 20 μm.