Figure 2. Loss of periostin does not negatively affect proliferation of adult murine dermal fibroblasts. Primary murine dermal fibroblasts were seeded at 2,000 cells/well in 24-well plates in 10% FBS supplemented media. Media was changed every 48 h throughout the course of the experiments. At the desired time-points, media was completely aspirated and the plate was frozen at -80°C. Once all time-points were captured, the CyQUANT cell proliferation assay kit (Invitrogen, C7026) was used to determine cell number as per the manufacturer’s protocol. A standard curve was used to obtain cell number. Error bars indicate standard error of the mean from four experiments, using four independent cell isolations.