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. 2012 Oct 22;7(10):e47259. doi: 10.1371/journal.pone.0047259

Table 1. Primers used for cloning and qPCR.

Primer name Sequence 5′-3′ Purpose, product size
pSAMRFFwd CAAAGGATCTTCTTGAGATCCTGGCTTCTGTTTCTATCAGCTGTCC Amplification of the p15A ori, 1062 bp
pSAMRFRev CGTGAGCATCCTCTCTCGTTTCGCGGGGCATGACTAACATGAGAA
dxs156Fwd CGCCGCACCGTCTGTGTCAT qPCR, 156 bp
dxs156Rev ATGGTTGTTGAGCGCGCCGA
bla136Fwd ACACCACGATGCCTGCAGCA qPCR, 136 bp
bla136Rev GCCGAGCGCAGAAGTGGTCC
aph134Fwd ACTCTGGCGCATCGGGCTTC qPCR, 134 bp
aph134Rev AACGTCTTGCTCTAGGCCGCG
cat110Fwd CGGATGAGCATTCATCAGGCGGG qPCR, 110 bp
cat110Rev AGACCGTTCAGCTGGATATTACGGC

qPCR  =  quantitative PCR.

bp  =  base pairs.

ori  =  origin of replication.

Fwd  =  forward primer.

Rev  =  reverse primer.