Table 1. Primers used for cloning and qPCR.
Primer name | Sequence 5′-3′ | Purpose, product size |
pSAMRFFwd | CAAAGGATCTTCTTGAGATCCTGGCTTCTGTTTCTATCAGCTGTCC | Amplification of the p15A ori, 1062 bp |
pSAMRFRev | CGTGAGCATCCTCTCTCGTTTCGCGGGGCATGACTAACATGAGAA | |
dxs156Fwd | CGCCGCACCGTCTGTGTCAT | qPCR, 156 bp |
dxs156Rev | ATGGTTGTTGAGCGCGCCGA | |
bla136Fwd | ACACCACGATGCCTGCAGCA | qPCR, 136 bp |
bla136Rev | GCCGAGCGCAGAAGTGGTCC | |
aph134Fwd | ACTCTGGCGCATCGGGCTTC | qPCR, 134 bp |
aph134Rev | AACGTCTTGCTCTAGGCCGCG | |
cat110Fwd | CGGATGAGCATTCATCAGGCGGG | qPCR, 110 bp |
cat110Rev | AGACCGTTCAGCTGGATATTACGGC |
qPCR = quantitative PCR.
bp = base pairs.
ori = origin of replication.
Fwd = forward primer.
Rev = reverse primer.