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. 2012 Oct 22;7(10):e47696. doi: 10.1371/journal.pone.0047696

Figure 5. Knockdown of UGT2B7 sensitizes melanoma cells to adriamycin and epirubicin treatment.

Figure 5

(A) Real-time PCR of UGT2B7 mRNA levels comparing melanoma cell lines stably expressing shRNA against UGT2B7 (WM115-2B7KD) or empty vector (WM115-pRS). (B) IC50 values determined from MTT assays assessing the contribution of UGT2B7 to melanoma resistance following treatment of temozolomide, adriamycin, epirubicin or vemurafeninb. (C) IC50 values determined from MTT assays assessing the sensitivity of melanoma cell lines with (WM115) and without (WM3211) UGT expression to anti-cancer drugs known to be metabolized by UGTs. All data graphs are the average of at least three independent experiments analyzed by GraphPad Prism. Error bars represent standard deviation and * indicates significance p<0.01 as compared to either control.