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. 2012 Oct 22;7(10):e47853. doi: 10.1371/journal.pone.0047853

Figure 2. Effect of cryptococcal laccase on the recruitment of total lung leukocytes and leukocyte subsets.

Figure 2

Lung leukocytes were isolated from mice infected with H99 or lac1Δ, antibody, stained and analyzed using flow cytometric analysis as per Materials and Methods. The total number of CD45+ leukocytes was determined by multiplying the frequency of CD45+ by total number cells at 2 and 3 wpi (A); Representative FACS plots show the staining strategy to isolate lung leukocyte subsets within gated populations of CD45+ leukocytess. Macrophage, Eosinophil, Neutrophil, and Lymphocyte subsets were identified based on the expression of Gr-1 vs CD11c. Total numbers of Macrophages, Eosinophils, Neutrophils, and Lymphocytse were determined by multiplying the frequency of each subset by the total number of CD45+ leukocytes at 3 wpi (B). Bars represent data (mean ± SEM) from 2–3 separate matched experiments, N = 6 and above for each of the analyzed parameters; *p<0.05 in comparison between wt and mutant; NS, no significant difference between wt and mutant.