Skip to main content
. 2012 Oct 22;7(10):e46631. doi: 10.1371/journal.pone.0046631

Figure 5. The antiviral activity of hepcidin is mediated by STAT3 activation.

Figure 5

(A) Western blot analysis for pSTAT3 and total STAT3 in Huh7.5 control cells and Huh7.5 cells incubated with hepcidin for 0.5 h, 1 h and 4 h. (B) Western blot analysis for STAT3 in Huh7.5-vector, Huh7.5-hepc, Huh7.5-antihepc stable cell lines with or without STAT3 siRNA transfection. Actin was used as the loading control. (C) qRT-PCR analysis for HCV mRNA expression in the cells as described in (B). RNA was prepared from these cells after infected by JC1 virus for 3 days. The data are presented as mean ± SD from three independent experiments.