Figure 7. Hepcidin induces a classic interferon-induced gene 2′-5′-oligoadenylate synthetase 1 (OAS1) and interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) expression.

Huh7.5 cells were infected by HCV-JFH1 virus or JC1 virus, and treated with or without hepcidin peptide for 48 hours. FLneo was also treated with or without hepcidin peptide for 48 hours. OAS1 mRNA levels were analyzed by qRT-PCR in these cells. Huh7.5 cells were infected by HCV-JFH1 virus, and treated with or without hepcidin peptide for 24, 48 and 72 hours. IFIT1 mRNA levels were analyzed by qRT-PCR. GAPDH was used as the internal control in the PCR reaction.