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. 1998 Nov;118(3):733–741. doi: 10.1104/pp.118.3.733

Figure 4.

Figure 4

Hydration of pollen grains and growth of pollen tubes on stigmas. A to D, Pollen on stigma papillae projecting through the exudate into air at the RH prevailing in the laboratory. Dry pollen (A and B) and pollen presoaked in exudate and drained (C and D) did not hydrate. Photographs were taken 1 h (A and C) and 18 h (B and D) after pollination. E and F, Pollen on stigma flooded with mineral oil at the start of the experiment and photographed 30 min and 4 h after pollination did hydrate. G and H, Growth of pollen tubes on stigmas held at controlled RH, photographed 16 h after pollination. G, Stigma at 88% RH. Only stigma papillae are visible above the exudate. H, Stigma at 100% RH. Pollen tubes have grown from the stigma into the surrounding air. pap, Papillar cell; pg, pollen grain; pt, pollen tube. Scale bar in A = 50 μm and refers to A through F; scale bar in G = 50 μm and refers to G and H.