Figure 3. Drosha negatively affects the steady-state level of endogenous Lin28 mRNA targets. PA-1 cells were transfected with siCon, siDrosha or siDicer. RNA and protein were extracted 72 h post-transfection and levels determined by RT-qPCR (A and C) and western blot analysis (B and D), respectively. In (A and C), RNA levels from siCon-transfected cells were arbitrarily set as 1. In (B and D), β-tubulin was used as loading control. Results are representative of three independent transfection experiments.