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Figure 2. Oxidation reaction of pep63. (A) DTNB’s assay of the pep63 after oxidation, UV-vis spectra of the for pep63 (_____) and pep63OX (- - -), the maximum at 412 nm indicates the presence of TNB2+ and the thyols; (B) RP-HPLC of the pep63 (a) and pep63OX (b) the molecular masses were obtained by ESI-MS of the corresponding peaks; (C) CD spectra of 50 μM pep63(____) and pep63OX (_ _ _) in 10 mM sodium phospate pH 6.2, buffer 25 mM KCl.
