Figure 2. Identification of genes in prostate cancer cells that respond uniquely to combined Wee1 and Hsp90 inhibition. (A) Expression profile of PC3 cells treated with Wee1 inhibitor (2.5 µM) for 24 h followed by 17-AAG (40 nM) for an additional 48 h. Single-agent treatments are shown for comparison; mRNA from each sample was subjected to microarray analysis. (B) Heat maps showing Survivin and Wee1 gene expression following single agent and combined treatments are shown. (C) Changes in Survivin and Wee1 expression shown by microarray analysis were confirmed by RT-qPCR. Error bars were generated based on a confidence interval of 95%. (D) Western blot analysis of Survivin, Wee1, Akt and phospho-Ser473 Akt proteins in PC3 cells treated as above. Hsp90 is shown as loading control.