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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Immunol. 2012 Sep 24;189(9):4537–4545. doi: 10.4049/jimmunol.1201111

FIGURE 5. IFN-β induces chemokines and enhances phagocyte killing of S. aureus.

FIGURE 5

A and B, BMDC were infected with WT-SA or ΔOatA-SA (MOI 10) and RNA was harvested at the indicated time points. CXCL10 (A) and CCL5 (B) mRNAs were measured by RT-PCR. C, BMDC were infected (MOI 10) with WT-SA plus 100 Units/ml recombinant murine IFN-β or vehicle control (-). Cells were lysed after 24 hours of infection and surviving intracellular CFU were plated and counted. D and E, Murine whole blood was treated with PBS (-) or recombinant murine IFN-β (final concentration 103 Units/ml) and mixed with WT-SA (final concentration 1×103 CFU/ml). Blood was incubated for 3 hours, and surviving bacteria were plated and counted (D). Representative images of PBS or IFN-β treated infected whole blood (E).