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. 2012 Aug 9;3(8):774–782. doi: 10.18632/oncotarget.577

Figure 4. Knocking down mutant IDH1R132C inhibits cell growth in vitro.

Figure 4

A, 50,000 cells with exogenous wild type IDH1 expression and inducible knockdown of endogenous IDH1 were seeded in 100mm cell culture dishes in triplicate and treated with 0 or 200ng/mL tetracycline. Cells were collected and counted daily for 5 days. Error bars represent one standard deviation. B, 50,000 cells with exogenous IDH1R132C expression and inducible knockdown of endogenous IDH1 were seeded in 100mm cell culture dishes in triplicate and treated with 0 or 200ng/mL tetracycline. Cells were collected and counted daily for 6 days. C, 300 cells were plated in 60mm cell culture dishes in triplicate. After 8-10 days treatment, cell colonies were stained with 0.05% crystal violet and counted. D, Indicated cell lines were treated with 0 or 200ng/mL for four days in triplicate and fixed. Cell cycle analysis was completed via FACS.