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. 2012 Aug 20;3(8):851–865. doi: 10.18632/oncotarget.586

Figure 1. GLI is the critical target in switching off HH survival signaling.

Figure 1

A: HT29 cells were treated with equimolar concentrations of GANT61 (20 μM), cyclopamine (20 μM) or GDC-0449 (20 μM) for 72 hr. The extent of cell death was determined at the end of drug exposure. B: HT29 or GC3/c1 cells were selected for resistance to cyclopamine or GDC-0449, respectively, by stepwise selection in increasing drug concentrations from 20 μM to 100 μM. Sensitivity of resistant cells to cyclopamine (100 μM), GDC-0449 (100 μM) or GANT61 (20 μM) was examined after 72 hr drug exposure. C: HT29 cells overexpressing GLI1cDNA or GLI2cDNA were examined for their sensitivity to GANT61 at increasing concentrations of 10 μM, 15 μM and 20 μM for 48 hr. Percent cell death was determined. Inset: Relative expression of GLI1 and GLI2 in HT29 cells overexpressing GLI1 (left) or GLI2 (right). Cell death was determined by Annexin V FITC/PI staining and FACS analysis. Data represent the mean +/− SD, n=2.