Figure 5. Localization and co-localization of γH2AX and MDC1 nuclear foci during DNA damage or during DNA repair following GLI1/GLI2 inhibition.

A: HT29 cells were treated with GANT61 (20 μM) for up to 48 hr (DNA damage), or B: for 24 hr followed by incubation in drug-free medium for 8 hr, 16 hr or 24 hr. γH2AX and MDC1 nuclear foci were examined by confocal microscopy as described in Materials and Methods and in the legend to Figure 2.