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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Immunol. 2012 Oct 1;189(9):4630–4639. doi: 10.4049/jimmunol.1102737

Figure 6.

Figure 6

STING-dependent IRF3 phosphorylation requires both calcium mobilization and ER stress. A) WT or STING-/- MEFs were pre-treated with 2μM MRT67307 for 30 min then 10μg/mL Tm for 6h as indicated. Cells were incubated with anti-pIRF3 (S386) followed by Alexa Fluor 488 secondary antibody and visualized by immunofluorescence microscopy. Results are representative of 4 independent experiments. B) WT or STING-/- MEFs were untreated (NT), treated with 1μM ionomycin or 2μM A23187 as indicated. Fixed cells were incubated with anti-pIRF3 followed by Alexa Fluor 488 secondary antibody and visualized by immunofluorescence microscopy. Results are representative of 3 independent experiments. C) Wild type or STING-/- MEFs were subjected to OGD (as in Figure 1) for 1h followed by 2 hours re-oxygenation. Cells were fixed (1C) or lysed (1D) and IRF3 nuclear translocation detected by immunofluorescence or western blot, respectively.