Fig. 8.

In vivo uptake of pCMV-β plasmid (A) and expression of β-galactosidase gene (mRNA level) (B) 24 h after pCMV-β, alone or coated on nanoparticles, was applied on a mouse skin area pretreated with microneedles. A 100 µl (20 µg pCMV-β) of each formulation was applied on C57BL/6 mice (8–10 weeks) at 0 h. pCMV-β recovered from the skin samples and the β-galactosidase mRNA level in the skin samples were determined 24 h later using qRT-PCR. MN, microneedles. Data reported are mean ± S.E.M. (n = 3). (*, p = 0.005, MN, (+) NP vs. MN, (−) NP; MN, (+) NP vs. MN, DNA).