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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: J Control Release. 2012 Sep 1;163(2):211–219. doi: 10.1016/j.jconrel.2012.08.027

Fig. 2.

Fig. 2

Transfection efficacies and cellular uptake of targeted and non-targeted polyplexes in the presence of inhibitors of endocytosis. M-3 cells were pretreated with chlorpromazine (CP), nystatin (N), filipin III (F), or their mixtures and targeted (PEI-PEG-MC1SP/DNA) or non-targeted (PEI-PEG/DNA) polyplexes with the plasmid encoding luciferase (A, B) or EGFP (C) reporter genes or plasmid DNA labeled with QD605 (D) for 4.5 h. Reporter gene expression was determined 48 h after transfection. Uptake of polyplexes was assessed by flow cytometry. Results are means ± S.E.M. (n = 4–6). *p < 0.05, **p < 0.01, ***p < 0.0001 (one-way ANOVA followed by a post hoc Dunnett’s t-test).