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. 2012 Oct 16;61(11):2967–2979. doi: 10.2337/db11-1824

FIG. 5.

FIG. 5.

Exendin-4 (Ex-4) decreased effect of Ang II on Erk1/2/PAI-1 signaling. A: Immunoblots of phospho–c-Raf (p-c-Raf)(Ser259), phospho–c-Raf(Ser338), and phospho-Erk1/2 (p-Erk1/2) in RGECs stimulated with Ang II in the presence or absence of PD98059, SB203580, exendin-4, or H89. **P < 0.001 vs. Ang II−/PD98059−/SB203580−/exendin-4−/H89−; †P < 0.05 vs. Ang II−/PD98059−/SB203580−/exendin-4+/H89−; ‡P < 0.05, ‡‡P < 0.001 vs. Ang II+/PD98059−/SB203580−/exendin-4−/H89−; ¶P < 0.05 vs. Ang II+/PD98059−/SB203580−/exendin-4+/H89−. B: Immunoblots of phospho–c-Raf(Ser259), phospho–c-Raf(Ser338), and phospho-Erk1/2 in RGECs stimulated with Ang II (10 nmol/L) in the presence or absence of exendin-4 or MDL12330A. *P < 0.05, **P < 0.001 vs. Ang II−/exendin-4−/MDL12330A−; †P < 0.05 vs. Ang II−/exendin-4+/MDL12330A−; ‡P < 0.05 vs. Ang II+/exendin-4−/MDL12330−; ¶P < 0.05 vs. Ang II+/exendin-4+/MDL12330−. One of three independently performed experiments is shown. Comparisons were made between groups using either two-sample and paired t tests for two-way comparisons or one-way ANOVA for multiple groups to establish statistically significant differences. Results are expressed as means ± SD. AU, arbitrary units.