FIG. 6.
Detection and quantitation of IGRP206–214 and JAK-1355–363 presented by NIT-1 β-cells. A: Flow cytometric analysis of Kd expression on NIT-1 cells treated with IFN-γ. B: NIT-1 cells (5 × 107) were treated with IFN-γ for 0–72 h and Kd peptide complexes immunoaffinity purified at each time point. Samples were spiked with AQUA peptides prior to HPLC separation and fractions subjected to liquid chromatography–MRM analysis. The amount of peptide is shown as copies/cell; values are triplicate biological replicates ±SEM. C: Kd MHC-peptide complexes were purified from a single pellet of 3 × 109 untreated NIT-1 cells for peptide quantitation. max, maximum; Un, untreated.