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FM clamping normalizes energy expenditure. Absolute (A) and average energy expenditure (B), RQ (C), and locomotor activity (D) of SD, HFD, and FMC mice was measured hourly. Mice from the SD and HFD group were fed with SD or HFD ad libitum. Mice from the FMC group were fed defined amounts of HFD to match FM to the SD group. All mice are male (n = 16 per group). All data are represented as mean ± SEM. ***P < 0.001.
