FIG. 5.

CR restores leanness independent of the diet. A: BW of mice that were fed with SD or HFD ad libitum in comparison with calorically restricted mice on SD or HFD. The SD and HFD groups were fed ad libitum, whereas the groups CR-SD and CR-HFD were calorically restricted and fed 50% of calories (given as SD or HFD) that were consumed on average in the SD group. FMC mice were fed defined amounts of HFD to match FM to the SD group. FM (B) and lean mass (C) curves of mice fed ad libitum or that were calorically restricted are shown. D: intraperitoneal GTT conducted after 6 h of fasting at day 45 of CR. E: Area-under-the-curve (AUC) analysis of GTT. F: C-peptide secretion at 30 min after intraperitoneal glucose challenge. All mice are male (n = 10–15 per group). All data are represented as mean ± SEM. Lowercase letters above bars (A–F) denote statistically similar (P > 0.05) groups.