FIG. 5.
Macrophages predominantly migrate to the pancreas and PLN. A: NOD mice (10–12 weeks of age) received 3 × 106 DiR-labeled M2r or M0 macrophages intraperitoneally. Mice were anesthetized prior to anterior imaging after 2 h and 1, 3, 6, and 8 days post–macrophage injection. Bright yellow or dark red represents high or low photon counts, respectively, and white arrows indicate injection sites. Two individual mice were analyzed, and the data represent two individual experiments. B: After transfer, the liver, kidney, spleen, pancreas, and PLNs were dissected after 3 days post–macrophage injection. The image is representative of eight individual mice (four treated with M0 and four with M2r). C: Photon count from each organ in B was quantified, and control organ photon count was subtracted to obtain the real macrophage emission. Error bars are presented in SEM. D: NOD mice received 2 × 106 DiI-labeled M2r macrophages (red) intraperitoneally, and pancreata were dissected at day 3 and stained for CD11b (green) and DAPI (blue). The data are representative of four different mice. (A high-quality digital representation of this figure is available in the online issue.)