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. 2012 Oct 16;61(11):2743–2752. doi: 10.2337/db11-1572

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 4. — 14-3-3 binding to TBC1D4 and site-specific phosphorylation in rested or exercised (Ex’d) vastus lateralis muscle in the saline and lipid trials. A: 14-3-3 binding to TBC1D4/TBC1D4 protein expression. B: TBC1D4 S318 phosphorylation/TBC1D4 protein expression. C: TBC1D4 S341 phosphorylation/TBC1D4 protein expression. D: TBC1D4 S588 phosphorylation/TBC1D4 protein expression. E: TBC1D4 T642 phosphorylation/TBC1D4 protein expression. F: TBC1D4 S704 phosphorylation/TBC1D4 protein expression. G: TBC1D4 S751 phosphorylation/TBC1D4 protein expression. H: Representative Western blots of TBC1D4 phosphorylation and 14-3-3 binding to TBC1D4 show the quality and signal obtained with the corresponding antibody and represent one subject; thus, they do not necessarily represent an exact mean of all subjects. Data are expressed as means ± SEM (n = 8). #P < 0.05 vs. saline trial; *P < 0.05, **P < 0.01, ***P < 0.001 vs. basal (0 min.) in respective trial; †P < 0.05, ††P < 0.01, †††P < 0.001 vs. rested leg. AU, arbitrary units.