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. 2012 Oct 16;61(11):2823–2832. doi: 10.2337/db12-0232

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© 2012 by the American Diabetes Association.

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FIG. 4. — Western blot analyses of liver and cultured cells. A: Western blot analyses of the livers from WT, vaspin Tg, vaspin^+/+, vaspin^+/−, and vaspin^−/− mice fed standard (STD) and HFHS chow. Upregulation of pAkt and pAMPK was noted in vaspin Tg mice, and pAkt and pAMPK were downregulated in vaspin^−/− HFHS mice. ATF6, pelF2α, and pIRE1α were upregulated in vaspin^−/− HFHS mice. B: In H-4-II-E-C3 cells, pAkt and pAMPK were enhanced by treatment of rhVaspin. C: Non-target shRNA control lentivirus transduction particles upregulated basal activity of pAkt; however, treatment of shRNA lentivirus for MTJ-1 and GRP78 suppressed pAkt in H-4-II-E-C3 cells. Impact on pAMPK was minimal by the treatment of shRNA lentivirus for MTJ-1 and GRP78. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.