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. 2012 Oct 16;61(11):2823–2832. doi: 10.2337/db12-0232

FIG. 5.

FIG. 5.

Knockdown experiments by siRNA and shRNA lentivirus for GRP78. A: Glucose tolerance test in WT and vaspin Tg HFHS mice and siRNA-GRP78 (n = 3). With the treatment of siRNA-GRP78, beneficial effects in glucose tolerance in vaspin Tg mice were not observed. B: Glucose tolerance test in WT and vaspin Tg mice fed HFHS chow and treated with siRNA negative control (siRNA-CTRL) (n = 3). *P < 0.05 vs. WT HFHS mice treated with siRNA negative control. C: Western blot analyses of liver tissues. D: The binding activity of 125I-vaspin using liver membrane fractions in vaspin Tg mice fed HFHS chow. Binding affinity of 125I-vaspin was not altered by the treatment of siRNA-GRP78 by Scatchard analysis. Bmax was reduced in the isolated liver membranes by the treatment of siRNA-GRP78. *P < 0.05 vs. siRNA negative control. E: Cell binding assay of 125I-vaspin using cultured H-4-II-E-C3. Bmax was reduced in H-4-II-E-C3 cells by the treatment of shRNA-GRP78 lentivirus. *P < 0.05 vs. shRNA negative control. Kd, dissociation constant.