FIG. 4.

DM+VEGFKO mice had severe glomerular injury and significant glomerular expansion. A: Light micrographs of glomeruli from each of the four treatment groups. At 7–8 weeks post–STZ injection, glomerular injury was evident in the DM+VEGFKO group only. Original magnification ×400. B: A lower-power view of a kidney in the DM+VEGFKO group demonstrates glomerular injury, proteinaceous casts in the tubules (*), and interstitial infiltrate. Original magnification ×200. C and D: Glomerular expansion was increased in DM+VEGFKO group at the late time point. E and F: Glomerular injury scoring showed DM+VEGFKO mice had significantly more injury at both early and late time points. In contrast, DM group alone showed less severe but significant glomerular injury compared with control group at the late time point. Early: WT (n = 2), DM (n = 3), VEGFKO (n = 4), and DM+VEGFKO (n = 3). Late: WT (n = 14), DM (n = 14), VEGFKO (n = 10), and DM+VEGFKO (n = 20). *P < 0.05, **P < 0.01, ***P < 0.001. (A high-quality digital representation of this figure is available in the online issue.)