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. 2012 Oct 16;61(11):2814–2822. doi: 10.2337/db12-0176

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 2. — Noncleavable Dlk1 regulates numerous genes during the G1-to-S-phase transition. A: Relative variation of gene expression in Dlk1_Total versus Dlk1_PS siRNA transfected 3T3-L1 preadipocytes at 48 h, as determined using GenMAPP software. Upregulated genes are magenta (P < 0.05) and purple (0.05 < P < 0.2), and downregulated genes are dark green (P < 0.05) and green (0.05 < P < 0.2). P values have been assigned to each gene after log2 transformation and a Student t test. B: Relative qRT-PCR of cell cycle–associated genes at 24–96 h after transfection of 3T3-L1 preadipocytes with DLK1_Total, DLK1_PS, or scramble siRNA. Significance was tested by a one-way ANOVA followed by a Bonferroni multiple comparisons test within each time point against scramble control. *P < 0.05; **P < 0.01; ***P < 0.0001. Raw data were normalized against multiple stably expressed reference genes (data not shown).