FIG. 4.

Chronic CNS infusion of OXM induces a sustained increase in BAT thermogenesis that requires a functional GLP-1R. ICV infusion of OXM induces a sustained increase in iBAT thermogenesis over the course of 6 days in WT mice (A), which is completely absent in the Glp1r^−/− mouse (B). The average increase over 5 days in iBAT temperature induced by OXM is significant in both the light and dark phases in WT mice (C) but not in the Glp1r^−/− mice (D). The ICV OXM–induced iBAT thermogenesis does not correlate with increased plasma T3, as measured at the end of the study (E). Food intake (F and G) and home cage locomotor activity (Loc. Act) (H and I) of WT and Glp1r^−/− mice during the period of ICV OXM infusion. Data are expressed as means ± SE (n = 6–8). *P < 0.05, **P < 0.01 vs. corresponding vehicle (VH); two-way ANOVA with Bonferroni post hoc test. KO, knockout; cnts, counts.