FIG. 5.

CNS infusion of OXM increases the expression of genes involved in thermogenesis in the iBAT of WT mice but not in Glp1r^−/− mice. PGC1a (A), UCP-1 (B), DIO2 (C), and FGF21 (D) gene expression in BAT is increased in WT but not in GLP-1R knockout mice after 3-day ICV OXM infusion. Similarly, ICV OXM increased the expression of genes involved in energy metabolism, such as MCT1 (E), GLUT-4 (F), LPL (G), AACS (H), FASN (I), CPT1B (J), PRDM16(K), and NRF1(L) in iBAT of WT mice. WT vehicle (VH)-treated mice were pair-fed with the WT OXM group. Data are expressed as means ± SE (n = 6–8). *P < 0.05, **P < 0.01, **P < 0.001 vs. corresponding vehicle; two-way ANOVA with Bonferroni post hoc test. KO, knockout; a.u., arbitrary unit.