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. 2012 Oct 23;2:762. doi: 10.1038/srep00762

Figure 3. tsl expression and patterns of dpERK in the prothoracic gland.

Figure 3

(A,B) A ring gland from a 3rd instar larva stained with anti-Tsl (red) and anti-Spectrin (green) antibodies and DAPI (blue). (A',B') Same pictures showing only Tsl accumulation; antibody signal is detected at the prothoracic gland (PG) and in the corpora cardiaca (CC) (arrowheads) but not in the corpus allatum (CA) (asterisk). (C) Specificity of the signal at the PG is shown by its absence in 3rd instar larvae mutant for a tsl null combination (Df(3R)cakiX-313/CASKX-307); conversely, signal at the CC appears to be unspecific because it is also detected in the tsl null combination (arrowheads). (D) A ring gland from a 3rd instar larva bearing a tsl-HA construct stained with an anti-HA antibody; signal is detected in the PG but not in the CA nor in the CC. All images correspond to single stacks. (E–G) Anti-dpERK staining (red) at the PG from wild type (E), torXR (F) and tsl4 (G) 3rd instar larvae; dpERK is detected only in the wild type.