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. 2012 Sep 24;109(41):E2794–E2802. doi: 10.1073/pnas.1205742109

Fig. 2.

Fig. 2.

Peripheral IRF4-deficient T cells share the innate phenotype of Itk−/− CD8+ T cells. (A) Splenocytes were isolated from WT, Itk−/−, and Irf4−/−(T) mice, and they were then stained with antibodies to CD4, CD8, CD44, and CD62L. CD4 vs. CD8 staining of total splenocytes (Upper) and CD44 vs. CD62L staining on gated CD8+ T cells (Lower) are shown. (B) Compilations of the total splenocyte numbers, percentages of CD8+ T cells, and absolute numbers of CD8+ T cells in the spleens are shown (n = 8). (C) CD8+ splenic T cells from WT, Itk−/−, and Irf4−/−(T) mice were analyzed for GFP, CXCR3, CD44, and CD122 expression. (D) CD8+ splenic T cells from WT, Itk−/−, and Irf4−/−(T) mice stained with antibodies to CD44, Eomes, and Tbet or were stimulated for 4 h with phorbol 12-myristate 13-acetone and ionomycin, and they were then analyzed for IL-4 vs. IFN-γ expression. Compilations of the data show the percentages of Eomes+ cells (n = 12), Tbet+ cells (n = 6–10), and IFN-γ+ cells (n = 10). Statistically significant differences are indicated by *(P < 0.05), **(P < 0.001), or ***(P < 0.0001) based on the Mann–Whitney test.