Fig. 3.

Restoration of the ∆bdlA dispersion phenotype to wild-type levels requires both truncated polypeptides of BdlA. (A) BdlA requires all conserved domains for functions, as constructs lacking any of the domains do not rescue the ∆bdlA dispersion-deficient phenotype. (B) Dispersion is restored in ∆bdlA upon coexpression of bdlA-PASa and bdlA-PASbTar from two separate vectors (∆bdlA/pJN-PASa/pMJT-PASbTar). Uninduced, no arabinose was added to growth medium; induced, arabinose was present. (C) BdlA oligomerization as determined using pull-downs depends on the presence of the PAS domains. A pull-down control (-) was performed in the absence of an HA-tagged bait protein. (D) PASbTarH interacts with PASa in PAO1/pJN-bdlA-V5 (P_BAD) as indicated using in vivo cross-linking and probing with anti-V5 antibodies. PAO1::P_bdlA-bdlA-V5 (P_bdlA) was used as control. Cross-linking with dithiobis(succinimidyl propionate) (DSP) was reversed using β-mercaptoethanol (β-ME).