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. 2012 Sep 24;109(41):16558–16563. doi: 10.1073/pnas.1206268109

Fig. 2.

Fig. 2.

Invasive behavior of breast cells and EMT genes is regulated by nicastrin. (A) Transwell invasion assay of HCC1806-ShLuc, HCC1806-ShNCT cells. Bars represent mean number of invaded cells ± SEM from three separate experiments, each in triplicate. *P = 0.001. (B) RT-qRT-PCR showing fold change of quantitative mRNA levels of MMP2, MMP9, Vimentin, Twist, Snail, and SIP1 genes in HCC1806-ShNCT versus HCC1806-ShLuc cells. (C) RT-qPCR showing fold change of quantitative mRNA levels of MMP2, MMP9, Vimentin, Twist, Snail, and SIP1 genes in MCF10ANCT versus MCF10A-Ctrl cells. GAPDH was used for normalization. Error bars are SEM of the fold change from three separate experiments, each in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001. (D) HCC1806ShNCT (2 × 105) cells were plated in a 6-cm Petri dish and transfected with 1.0 ng of full-length NCT cDNA (SC100655; Origene) and the pCMV6-XL4 empty vector control, using Lipofectamine 2000 (Invitrogen). Cells were incubated for 24 h and then lysed for mRNA and protein extraction. (A) RT-qPCR of NCT, Vimenti, and Snail showing a marked increase of relevant gene mRNA levels. *P < 0.01. GAPDH was used for normalizing. Error bars represent SD of two separate experiments.