Fig. 2.

PAR₂-induced recruitment of leukocytes to the lungs requires β-arrestin-2. (A–C) Bar graphs depict cell numbers in BALF: total cells (A), eosinophils (B), and lymphocytes (C). Numbers from all OVA-treated mice were statistically different from saline controls except eosinophils in β-arrestin-2^−/− treated with OVA+2fAP. (D) Flow-cytometric determination of the percentage of CD45⁺ cells that were lymphocytes, eosinophils, or other cells. Significant differences between bracketed groups are indicated as follows: ⁺P < 0.05, ⁺⁺P < 0.01, and ⁺⁺⁺P > 0.001. (E) Representative scatter plots of flow-cytometric analysis of BALF cells. High FSC/SSC, CD45⁺ granulocyte populations (G) were analyzed for the presence of CD11b and CCR3 [eos (E)]. Low SSC, CD45⁺ lymphocyte (L) populations were further analyzed for the presence of the T-lymphocyte marker, CD3 (L*) (n = 18 for saline, n = 24 each for OVA+CP and OVA+2fAP).