Fig. 2.

ORMDL3 transfection into lung epithelium regulates expression of metalloproteases, chemokines, and OAS genes. (A) Lung epithelial cells (A549) transfected with GFP tagged full-length ORMDL3 (green color; blue nuclei). (B–D) qPCR was performed on primary human bronchial epithelial cells transfected with either full-length ORMDL3 or with the empty vector control. The genes that were highly induced by ORMDL3 transfection include metalloproteases (B), chemokines (C), and OAS (D) genes. Immunohistochemistry was used to demonstrate increased MMP-9 (E) and OAS-2 (F) expression in airway epithelium in vivo in WT mice challenged with OVA.