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. 2012 Sep 17;109(41):16504–16509. doi: 10.1073/pnas.1212797109

Fig. 3.

Fig. 3.

ClsC needs YmdB to fully complement CL synthesis. Cls were expressed from plasmid pBAD30 in ∆clsABC strain BKT12, induced with 0.2% arabinose, and grown to stationary phase. (A) One-dimensional TLC developed with solvent 4 followed by charring revealed clsA (pBAD30-A) and clsB (pBAD30-B) restored CL production, whereas clsC (pBAD30-C) could only produce a trace amount of CL; no CL was detected in the vector control (VC). Coexpression from the same promoter (pBAD30-YC) of both ymdB and its neighbor clsC restored CL levels to higher levels than clsC by itself. (B) Lipid extracts from strains carrying plasmid pBAD-C or pBAD-YC were analyzed by LC/MS/MS. Vector control showed no CL production, whereas clsC expression alone only produced trace amounts of CL, with its doubly charged ion peaks being magnified in the boxed inset. Expression of ymdB-clsC resulted in high levels of CL production (Lower).