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Mutations in the HKD motifs of ClsC block CL synthesis. The WT without a plasmid or ∆clsABC strain carrying vector control (VC), pBAD-YC H130A, or pBAD-YC H369A (indicating mutation in ClsC HKD motif) was grown to stationary phase in the presence of 0.2% arabinose. TLC analysis of lipid extracts using solvent 4 and charring showed no increase in the CL spot over the vector control for ClsC, in which either HKD motif was mutated but PG was elevated.
