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LAMP reactions. (A) 2% agarose gel electrophoretic analysis of a typical 75 min LAMP reaction. Lane a: 1 kb DNA ladder (Invitrogen). Lanes b to k: LAMP products stemming from a M13mp18 target introduced at 0, 6.5, 32.5, 65, 165, 325, 650, 1625, 3250, and 32500 copies, respectively, per 25 μL reaction mixture. (B) Time-course of CHA detection via fluorescence. Inset: Reaction rate (change in fluorescence/min) as a function of copy number of the M13mp18 template.
