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Proof-of-concept experiments of coupling LAMP to AND gate dual-CHA circuit proof-of-concept experiments. (A) Scheme for the AND gate pathway. (B) True value table of AND gate. (C) Time-course of AND gate using C1 and C2 as two inputs. (D) Time-course of dual-CHA response with (red) and without (black) LAMP template. [C1]=[C2] =8 nM, [B-acceptor1]=[F-acceptor2]=12.5 nM, [H1]=[H3]=50 nM, [H2]=[H4]=400 nM, [F3]=1/2[Q]=1/2[M]=50 nM.
