Fig. 6.

The effects of hda19-1 and ap2-2 mutation on histone acetylation levels. (A) Histone acetylation modifications H4K16Ac, H4K5Ac and H3K9Ac are increased in hda19-1 Arabidopsis flowers compared with wild type (left). H4K16Ac levels in ap2-2 flowers are comparable to that of wild type (right). Band intensities were normalized relative to total histone H3 loading controls. (B) Anti-H4K16Ac ChIP showing enrichment at the second intron of AG and at two promoter regions of SEP3 in ap2-2 flowers. A slight enrichment is seen at the promoter of AP3. Data are represented as mean ± s.e.m. of three biological replicates and are presented as the ratio of enrichment in ap2-2 over that of wild type. Data were normalized relative to INPUT and ACT2 abundance. The constitutively expressed PP2A gene serves as a negative control. Student's t-test was used to determine the significance of target enrichment relative to PP2A (*P≤0.08; **P≤0.05).