Fig. 7.
Effects of β-catenin deletion on mouse mammary mesenchyme. (A-N) Histology (A,B), DAPI staining to demonstrate muclei (C,D), efficiency of cre-mediated recombination (E,F) and analyses of mesenchyme marker expression (G-N) in a representative mammary bud from a Dermo1-cre;tdTomato (A,C,E,G,I,K,M) and a Dermo1-cre;tdTomato;β-cateninlox/lox (B,D,F,H,J,L,N) embryo at E13.5. Note paucity and lack of organization in the mesenchyme in B compared with compacted, concentrically arranged fibroblasts in A. Also note mosaic expression of Dermo1-cre as demonstrated by the GFP reporter (F) and corresponding reduction of β-catenin (H) and all tested mesenchyme markers (J,L,N) in the regions in which Dermo1-cre efficiently deletes β-catenin (asterisks). Dotted lines delineate the dermo-epidermal junction and the mammary bud. Scale bars: 100 μm.