Fig. 9.
Loss of mesenchymal β-catenin impairs sexual dimorphism. (A-H) Histology (A,B), TUNEL staining (C,D) and immunohistochemistry for AR (E,F) and ER (G,H) on mammary buds from E14 male WT (A,C,E,G) and Dermo1-cre;β-cateninlox/lox (B,D,F,H) embryos. Note that although some mammary mesenchyme is present in Dermo1-cre;β-cateninlox/lox (B, arrow), the bud remains connected to the epidermis via a stalk, there is a lack of mesenchymal apoptosis (D, white arrows) and reduction in AR and ER staining (F,H). Dotted lines delineate the dermo-epidermal junction and the mammary bud. Scale bars: 100 μm.