Table I.
Phenotypic evaluation of 20-oxidase-overexpressing lines
| Line | PCR Result | Plants | Terminal Heighta | Average Internode Lengthb | (kanr/kans)c | χ2 (P at 3:1) |
|---|---|---|---|---|---|---|
| no. | cm | |||||
| 25-1 | + | 17 | 39.6 ± 1.3 | 0.98 ± 0.03 | 106 /36 | 0.01 |
| − | 11 | 34.3 ± 0.9 | 0.85 ± 0.04 | (0.80 –0.50) | ||
| 25-2 | + | 31 | 41.1 ± 0.4 | 0.98 ± 0.01 | 119 /48 | 1.25 |
| − | 10 | 30.2 ± 1.1 | 0.84 ± 0.02 | (0.50 –0.20) | ||
| 25-3 | + | 17 | 45.1 ± 0.6 | 0.97 ± 0.02 | 150 (40)d/55 | 0.84 |
| − | 4 | 33.5 ± 1.9 | 0.84 ± 0.03 | (0.50 –0.20) | ||
| 25-24 | + | 25 | 43.6 ± 1.1 | 0.96 ± 0.02 | 216 /79 | 0.50 |
| − | 6 | 36.5 ± 0.6 | 0.85 ± 0.02 | (0.50 –0.20) | ||
| Wild type | 20 | 33.6 ± 0.6 | 0.90 ± 0.00 | |||
T2 seeds from each of the four T1 lines were planted, and leaf samples collected from each T2 plant were tested for the presence of the transgene by PCR. Each plant from the segregating T2 population was placed into one of two groups, depending on the presence or absence of the transgene. The physical characteristics were then measured and compared between these two groups for each line. Data are means ± se.
Height at which the main stem produced at global proliferative arrest.
Terminal height/number of branches (lateral shoots plus siliques).
Media-grown T2 seedlings were assayed for the presence or the absence of the selectable marker kanamycin by assaying for the bleaching effect of this antibiotic on early seedlings. kanr, Kanamycin-resistant; kans, kanamycin-sensitive.
Of the 150 kanr seedlings listed in this line, 40 were albino, possibly as a result of a recessive mutation caused by T-DNA insertion.